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The cholinergic neurons from the PPN and LDT control equally SNc and VTA neurons by way of activating acetylcholine receptors in these neurons [eight]. Combining optogenetic and Mind slice patch-clamp procedures, Xiao et al. [8] shown that five–10 s of optogenetic stimulation of cholinergic projections in the PPN and LDT evokes inward currents
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